The results of live and dead test of cells detected by TailTM Image-Based Cytometer.

Green column represented the number of live cells and red column represented the number of dead cells. (A) The raw data of fluorescence response of T24 cells after treated with different concentrations of 19-BJB for 48 h (0, 0.5, 1, 2, 4, 8 μM) was showed. (B) T24 live cells were stained green by 4 μM calcein AM and dead cells were stained red by 2 μM ethidium homodimer. The histogram data (C) of T24 are expressed as means ± SD from triplicate samples for each treatment. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.