The results of live and dead test of cells affected by 19-BJB and ATMI, detected by TailTM Image-Based Cytometer.

Green column represented the number of live cells and red column represented the number of dead cells. Photos of T24 live cells were stained green by 4 μM calcein AM and dead cells were stained red by 2 μM ethidium homodimer. The raw data of fluorescence response were showed (A). Cells were treated by two ways, one for treated with 19-BJB for 48 h (0, 4, 8 μM), the other treated with 10 μM ATMI first, then 19-BJB for 48 h (4, 8 μM). The histogram data (C) are expressed as means ± SD from triplicate samples for each treatment. ****p < 0.0001.