pone.0271654.g004.tif (7.09 MB)
The reconstructed cryo-EM map.
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posted on 2023-01-04, 18:22 authored by Sathya Srinivasachari, Vikas R. Tiwari, Tripti Kharbanda, Ramanathan Sowdamini, Ramaswamy SubramanianThe crystallographic hexamer model was fit into the map. The model fits the map well and confirms the hexameric nature of HiNagB.
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terminal helix implicatedmd simulations indicatelacks allosteric regulationhexamers &# 8212less stabilizing energydiv >< pconverting sialic aciddimer interface suggestsenzyme nagb existssialic acidenergy sourcetrimeric interfacedimeric interfacedimer interfacestructural basispasturella multocidaoligomeric variationsoligomer formationnegative pathogensnagb dimernagb containlast stephaemophilus influenzaeevolutionary linkcrystal structurescomputational modelingallosterically regulated>- nagb
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