The myosin inhibitors, myoVin1 or para-aminoblebbistatin, do not affect viral replication or viral protein distribution in the cytoplasm of the cell body following treatment of axons.

Neuronal cultures in the cell body compartment were HSV-1 or mock-infected and the axonal compartment was treated with either myoVin1 or para-aminoblebbistatin (pBlebb) at 6 hpi. Neuronal cultures were fixed at 30 hpi and immunostained for HSV-1 C capsids with either myosin V or myosin II. Cultures were examined using a Leica SP5 II confocal microscope. Micrographs of HSV-1 infected neurons showing label for viral envelope (green), viral capsid (red) and myosin V (cyan) following inhibition of myosin V by myoVin1, or myosin II (cyan) by para-aminoblebbistatin. Dashed lines outline the nucleus. Scale bars = 10 μm.

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