ppat.1009855.g003.tif (2.83 MB)

The majority of SHIV-1157ipd3N4 infected cells are found in the small intestine 96 hours after oral challenge.

figure

posted on 2021-11-18, 18:56 authored by Roslyn A. Taylor, Michael D. McRaven, Ann M. Carias, Meegan R. Anderson, Edgar Matias, Mariluz Araínga, Edward J. Allen, Kenneth A. Rogers, Sandeep Gupta, Viraj Kulkarni, Samir Lakhashe, Ramon Lorenzo-Redondo, Yanique Thomas, Amanda Strickland, Francois J. Villinger, Ruth M. Ruprecht, Thomas J. Hope

Fluorescent microscopy and quantification of SHIV-1157ipd3N4 infected cells from eight animals that were sacrificed at 96 hours after dual viral oral challenge. Tissues were dissected, analyzed by IVIS, frozen, and tissue blocks with high luciferase activity were cryosectioned. Slides were stained for CD3, CCR6, AG3, and Hoechst. Representative fluorescent microscopy images. A-B) Cryosections of the small intestine showing SHIV-1157ipd3N4 infected cells A) 40X panels, scale bars 60 microns B) 100x panels, scale bars 20 microns C) Quantification of total number of SHIV-1157ipd3N4 infected cells by tissue type. Each dot represents the total number of cells found in five 40x panels in one individual animal.