posted on 2024-03-14, 17:47authored byHuifang Qiu, Yuanhao Li, Min Yuan, Huali Chen, Ajai A. Dandekar, Weijun Dai
PlasR-GFP (A), PrhlA-GFP (B) and PpqsA-GFP (C) reporter plasmids were mobilized into target strains. Strains bearing reporters were grown in casamino acids medium at 37°C. The expression level of GFP in each strain was quantified using a microreader and reported as relative fluorescence units divided by OD600. WT, wild-type PAO1; ΔpsdR, PsdR-null mutant; ΔpsdR::PrrnB::psdR, PsdR-null mutant carrying a single copy of psdR driven by a rrnB promoter. Data of indicated strains are presented as mean ± SD (n ≧ 4).