The effect of extracellular 4HNE on human bronchial epithelial cells.

(A,B) The effect of extracellular 4HNE on the activities of cellular TrxR/Trx. The method used measures the total activity of TrxR/Trx. (C,D) The effect of extracellular 4-HNE on the transcription of TrxR1/Trx1. (E,F,G) Representative Western blots (left panel) and relative density values (right panel) for the bands in the left panel. GAPDH was used as an internal reference. In the bar graphs, the values are expressed as mean ± S.D. (n ≥ 3). (H) Cell viability was affected by 4HNE concentration and incubation time. *, P < 0.05; **, P < 0.01 versus control without 4HNE treatment.