ppat.1009425.s002.tif (284.76 kB)
The Tat inhibitor Bayer 11–7082 has no effect on light output at 20 or 40 μM from a constitutive P. aeruginosa CTX::tac’-luxCDABE chromosomal reporter fusion.
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posted on 2021-08-30, 17:38 authored by Eliza Ye-Chen Soh, Frances Smith, Maxime Rémi Gimenez, Liang Yang, Rebecca Munk Vejborg, Matthew Fletcher, Nigel Halliday, Sophie Bleves, Stephan Heeb, Miguel Cámara, Michael Givskov, Kim R. Hardie, Tim Tolker-Nielsen, Bérengère Ize, Paul WilliamsThe co-solvent DMSO, had no effect at 0.4 or 0.8% on the lux reporter fusion Data are presented as maximal light output as a function of growth (RLU/OD600). Experiments were repeated in triplicate at least twice.
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wild type levelsreduced bioluminescence revealedreduced antibiotic tolerancedependent quorum sensingdependent autoinduction resultingdiv >< pdefective biofilm architecture>< sub ><primary signalling defectidentify factors requiredpseudomonas aeruginosa tat prevented pqs>< sub xmlnsedna deficient biofilmsaeruginosa tat aeruginosa dependent qsp biofilm maturationtat tat substratesub xmlnsrieske sub>rhla pqse pqsa pqs phza1 lux cytc cytb 1 transposon librarysulfur subunitrieske ironqs ).parent strainmembrane vesiclemajor constituentgenetic complementationgenes resultsextracellular matrixextracellular dnaexogenous pqsenhanced susceptibilitydriven autoarginine translocationalso phenocopied34 validated
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