The HMR C-terminus is required for HMR localization in proximity to centromeres and HP1a-bound chromatin (related to Fig 4).

(A) Heatmaps of ChIP-seq profiles (z-score normalized) centred at high confidence FLAG-HMR peaks in 4 kb windows. Peaks are grouped by HP1a class and sorted by the ChIP signal in native HMR ChIP. From left to right, anti-HMR ChIP in untransfected cells, anti-HMR ChIP in cells transfected with FLAG-Hmr⁺ and FLAG-Hmr^dC, anti-FLAG ChIP of cells transfected with FLAG-Hmr⁺ or FLAG-Hmr^dC, and anti-HP1a and anti-CP190. The latter two are representative of the two classes of HMR peaks: HP1a-proximal and non-HP1a-proximal. (B) Chromosome-wide FLAG-HMR ChIP-seq profiles (z-score normalized) for Hmr⁺ (light blue), Hmr^dC (dark blue) and HP1a (green). Chromosomes X and 4 are shown. (C) Hmr^dC is depleted at heterochromatin rich chromosome 4. Percentage of FLAG-HMR ChIP-seq peaks located on chromosome 4 for each replicate (n = 5). Hmr⁺ (light blue) and Hmr^dC (dark blue) are shown. P-values are obtained by a linear model. FLAG-HMR plots represent an average of 5 biological replicates.

(TIFF)