ppat.1011831.s004.tif (3.18 MB)
TgPP6C deletion has no notable effects on the structure of T. gondii rhoptries, dense granules, apicoplast, mitochondria or micronemes.
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posted on 2023-12-13, 18:35 authored by Qin-Li Liang, Lan-Bi Nie, Hany M. Elsheikha, Ting-Ting Li, Li-Xiu Sun, Zhi-Wei Zhang, Meng Wang, Bao-Quan Fu, Xing-Quan Zhu, Jin-Lei WangIMC1 (green) and DAPI (blue) stains were used to denote the parasites and nuclei (DNA), respectively. Anti-ARO, anti-GRA32, anti-CPN60, anti-HSP60 and anti-MIC2 antibodies were used to stain the rhoptries, dense granules, apicoplast, mitochondria and micronemes, respectively. Scale bars: 2 μm.
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six conserved motifsphosphoproteomic analysis revealedfunctional studies showeddiv >< pcell cycle progressiontgpp6c significantly reducedtgpp6c significantly attenuatedtgpp6c predominately alteredparasite lytic cycleparasite cell cycletoxoplasma gondii cell divisiontoxoplasma gondii tgpp6c contributesparasite ’parasite growthtranslational regulatorstaken togethersynchronous divisionresults showpys tachyzoitespru cystsproteins involvedprotein phosphatasesphosphorylation statuslow homologylethal dosedisordered rosettesdeficient typedaughter cellscomplex whereasbradyzoite differentiation
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