TcdB variants have different receptor preference in HeLa cells.

(A-D) The sensitivities of HeLa wildtype (WT), FZD1/2/7^–/–, and CSPG4^–/–cells to TcdB1 (A), TcdB2 (B), TcdB3 (C), and TcdB4 (D) were measured using the cytopathic cell-rounding experiments (n = 6). (E) The change of toxin resistance in different cell lines was quantified and normalized to the WT. Error bars indicate mean ± standard deviation. (n = 6, *P<0.001, n.s. = not significant, versus the WT, student’s t-test.).