ppat.1008937.g002.tif (1.35 MB)
Targeted sequencing of PA and PB1 using NGS showed the percentage of PB1 K229R and PA P653L mutations for donor, contact and indirect contact ferrets.
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posted on 2021-06-01, 17:41 authored by Daniel H. Goldhill, Ada Yan, Rebecca Frise, Jie Zhou, Jennifer Shelley, Ana Gallego Cortés, Shahjahan Miah, Omolola Akinbami, Monica Galiano, Maria Zambon, Angie Lackenby, Wendy S. BarclayThe top pie chart shows the percentage of each genotype for residue 229 in PB1 with the mutant (R229) in red and the wild type (K229) in black. The bottom pie chart shows the percentage of each genotype for residue 653 in PA with the mutant (L653) in blue and the wild type (P653) in black. The inoculum shows 5% K229 and 5% P653. For each infected ferret, two sequenced time points (as described in Fig 1) are shown. The group 4 indirect contact was not infected.
