Tamoxifen reduces AMCase and acid production in stomach which limits Ascaris larval migration.

(A) BALB/c mice were pretreated with tamoxifen intraperitoneally before challenged by oral gavage with 2,500 eggs of Ascaris, in PBS or vehicle containing recombinant AMCase at pH 2. Reduction of (B) AMCase and (C) H⁺-K⁺ ATPase was confirmed by qPCR. (D) Larval isolated from the liver 4 days post infection were quantified. (n≥4, mean±S.E.M, *p<0.05, **p<0.01, ****p<0.0001 using two-tailed Student’s t-test (B, C) or one-way ANOVA followed by Bonferroni’s test for multiple comparison (D). Data are shown as representative of two independent experiments. Illustration generated by Biorender.com).