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T cells in the blood.

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posted on 2021-12-20, 18:44 authored by Alexandra Melton, Lara A. Doyle-Meyers, Robert V. Blair, Cecily Midkiff, Hunter J. Melton, Kasi Russell-Lodrigue, Pyone P. Aye, Faith Schiro, Marissa Fahlberg, Dawn Szeltner, Skye Spencer, Brandon J. Beddingfield, Kelly Goff, Nadia Golden, Toni Penney, Breanna Picou, Krystle Hensley, Kristin E. Chandler, Jessica A. Plante, Kenneth S. Plante, Scott C. Weaver, Chad J. Roy, James A. Hoxie, Hongmei Gao, David C. Montefiori, Joseph L. Mankowski, Rudolf P. Bohm, Jay Rappaport, Nicholas J. Maness

A-B. CD4+ (A) and CD8+ (B) T cell frequencies in the blood before and 6-, 14-, and 21-days post SARS-CoV-2 infection. C-D. Changes in Ki-67 expressing CD4+ (C) and CD8+ (D) T cells. Bars represent median E. tSNE plots displaying changes in PD-1 expression (red) in peripheral CD45+ cells overtime. MA24 (E) and MA28 (F) displayed as representative animals. G. Merged tSNE indicating phenotype of the tSNE defined cell populations in E and F. H. Average changes in the percentages of CD4+, CD8+, CD4- CD8- (DN) and CD4+ CD8+ (DP) T cells within the total PD-1+ CD3+ cell population. I-L. Frequency of PD-1+ expressing CD4+ (I), CD8+ (J), DP (K) and DN (L) T cells in the blood. Bars represent median. Kruskal-Wallis comparison of overall means (PKW) and Dunn’s Multiple comparisons (designated by line, PD) tests used to determine significance. P values ≤0.05 reported. Baseline (-7): n = 4, Day 6: n = 4, Day 14: n = 2, Day 21: n = 2.

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    severe cardiovascular symptomsmacaca nemestrina highly transmissible variantsexpress cytotoxic moleculesdiv >< palso noted increasesthus may servesiv ), includingpulmonary pathologic lesionspredominantly cd4 +.pigtail macaque (<respiratory viral challengeuseful animal modelsimian immunodeficiency virussevere disease coursenovel coronavirus sarsreveals new immunereveals newincluding cd4thus proposedpulmonary infiltratespigtail macaquesimmune dynamicsanimal models>) modelviral dynamicsupregulate cd8understanding mechanismstesting vaccinesstudying pathogenesisneutralizing antibodiesinfections risingextensively studieddecember 2021data demonstratecomplex signaturescoagulation markerscausative agentassessing sars>) demonstrate2 viral

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