ppat.1011859.s002.tif (4 MB)

TEM demonstrated the infection of follicle and nurse cells by N. bombycis.

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posted on 2023-12-07, 18:49 authored by Chunxia Wang, Bin Yu, Xianzhi Meng, Dan Xia, Boyan Pei, Xiangyou Tang, Guizheng Zhang, Junhong Wei, Mengxian Long, Jie Chen, Jialing Bao, Chunfeng Li, Guoqing Pan, Zeyang Zhou, Tian Li

(A, B) TEM demonstrated the infection of FC by N. bombycis. (C, D) TEM analysis of NCs infected by N. bombycis. (E, F) TEM showed the infection of oocyte by N. bombycis via the FC. (G, H) TEM showed the infection of oocyte by N. bombycis from the NC. (I, J) N. bombycis in the oocyte was distributed around the yolk granules. The yellow dashed lines indicate the boundaries of cells. The arrow indicates the N. bombycis; spores were stained using FB28 (blue); N. bombycis in proliferation was labeled with anti-N. bombycis polyclonal antibody conjugated with Alexa Fluor 488 (green); nuclei were stained by PI (red). N, nucleus; S, spore; ESP, empty spore shell; OS, ovariole sheath; FC, follicular cell; O, oocyte; NC, nurse cell; Mr, meront; Sp, sporont; Sb, sporoblast.