Survivability is decreased in zebrafish embryos following exogenous treatment with an amino-terminal fragment of nApoE4_1-151.

A. Embryos at 24 hpf (prim-9 stage) were segregated into three groups: controls (untreated), 25 μg/ml or 50 μg/ml nApoE3_1-151, and 25 μg/ml or 50 μg/ml nApoE4_1-151. Embryos that lacked a heartbeat for 10 seconds were stimulated to induce movement. If no movement or heartbeat was detected, embryos were considered to be non-viable. Significant mortality was observed at both concentrations of nApoE4_1-151 by 48 hpf (orange and green dotted lines) compared to non-treated controls (orange dotted line) or nApoE31-151 (blue dashed line and black dotted lines). N = 3 independent trials, N = 5 fish/treatment. B. The Mosaic Plot depicts mortality based on a lack of heartbeat and response to physical stimuli following exogenous treatment of 48 hpf zebrafish embryos with either 25 μg/ml nApoE3_1-151 or nApoE4_1-151 for 24 hours. The blue filled region of the bar graph designates 25 μg/ml treatment of nApoE4_1-151 which led to a significant portion of the embryos being designated as dead. The red dotted portion of the bar graphs indicates less than expected were alive (p = 1.17e-13). All blank cells indicate the sample group followed the estimated trend. Data indicated significant morality for only the nApoE4_1-151 group. N = 3 independent experiments, 15 embryos/treatment.