Suppression of angiogenic cellular process by a repebody.

(A) Effect of r-C2 on VEGF-induced proliferation of HUVECs. Cells were incubated with VEGF (20 ng/mL) in the presence of 2.5 μM of either a repebody or bevacizumab for 48 hrs. *P < 0.05 compared with incubation using VEGF only. (B) Inhibition of VEGF-induced cellular migration by r-C2. HUVECs on the plate were scratched using a needle and incubated with VEGF in the presence of either a repebody or bevacizumab. The dotted line indicates a scratched area. The number of cells that migrated across the line were counted and analyzed. This experiment was performed in triplicate. *P < 0.05 compared with incubation using VEGF only. (C) Images of endothelial cellular migration assay. HUVECs were stained with a 0.05% crystal violet solution.