Supernatant transfer assay for the determination of soluble factors involved in MNGCs formation in bMϕ.

(A) Supernatant transfer assay from bMϕ (non-infected (NI), infected with Mtb or Mbv), or from hMϕ infected with Mtb or Mbv for 24 h, onto naïve bMϕ. A fraction of supernatant from bMϕ-infected with Mbv was heat inactivated and used to stimulate naïve bMϕ (heat Mbv). Graph represents the number of MNGCs formed in cultures of naïve bMϕ following the addition of 400 μl of supernatant derived from cultures of bMϕ infected with Mbv or Mtb (B) Supernatant transfer assay from non-infected hMϕ (NI), hMϕ-infected with Mtb, Mbv, or bMϕ infected with Mtb or Mbv for 24 h, onto naïve hMϕ. A fraction of supernatant from hMϕ-infected with Mbv was heat inactivated and used to stimulate naïve hMϕ (heat Mbv). The graph represents the quantification of MNGCs for each condition tested. (A and B) Data are representative of two independent experiments, each carried out in duplicates.

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