Sup4v2

Supplementary information 4. Mean relative abundances of 5S, 18S and 28S rRNA and their geometric means in ovaries from beluga sturgeon, Huso huso at different maturity stages. RNA abundances were normalized over µg total RNA (‘relative copy number’), as determined by Ribogreen. A: 5S rRNA: 5S ribosomal RNA; B: 18S rRNA: 18S ribosomal RNA; C: 28S rRNA: 28S ribosomal RNA; D: geometric means of expression of three ribosomal RNA genes. Different letters above bars indicate significantly different means (One-way ANOVA, P < 0.05). Abbreviations for gonadal development: PN: perinucleolar (n = 7); OD: oil droplet (n = 19); PY: primary yolk (n = 4); SY: secondary yolk (n = 3) and TY: tertiary yolk (n = 3).