Subclasses/isotypes are tuned differentially across vaccine regimens.

Plasma samples were assayed with a customized Luminex assay against gp140 ConS antigen and IgG1, IgG3, IgA1 and IgA2 relative titers in mean fluorescence intensity (MFI) are depicted here. A: The violin plot shows relative antibody titers for each vaccination arm for 5 HVTN trials at the peak immunogenicity time point, lines are indicating median and quartiles. The dotted line indicates placebo levels (averaged across all trials). IgG1, IgG3, IgA1 and IgA2 were measured against gp140 ConS. B: The radar plot shows combined IgG1, IgG3, IgA1 and IgA2 data, data for each subclass were z-scored. Colors correspond to the different trials as indicated in legend. C-D: Each line plot shows IgG1 (C) and IgG3 (D) levels for each trial over time starting at baseline (B), post prime (PP), post-boost (PB) and durability (D) time point. For trial 078, PP indicates Month 1, after the first but before the second prime administration. Different trial arms are depicted in different colors, placebo is indicated in black for panel A.