pone.0240765.s002.tif (544.54 kB)
Steps on cell segmentation for phase-contrast images of MDA-MB-231 breast cancer cell lines.
figure
posted on 2021-07-13, 17:25 authored by Jianchen Yang, Jack Virostko, David A. Hormuth II, Junyan Liu, Amy Brock, Jeanne Kowalski, Thomas E. YankeelovThe size of the whole well image is 2400 x 2400 pixels. Here we present a window of 400 x 400 pixels from an example image. Panel A: raw image of MDA-MB-231 cells; Panel B: image post binarization; Panel C: image post ‘imclose’; Panel D: image post ‘bwareaopen’, the final cell mask; Panel E: overlay of raw images and the cell mask for MDA-MB-231 cells.
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