Simulations showing the dependency of the Potential of death induction (P_death) on temporal and spatial features.

A. Experimental data showing the spatial localization of death events at different time points (above), and the corresponding P_death measurements (below) on a video of MDA-MB-231 cells treated with 1 μM doxorubicin (the same reported in Fig 6A). B. Simulation of a video with the same death events as in A, but with a spatially random distribution, maintaining approximately the relative object distances. Note that the corresponding P_death measurements are increasing much less than in A, indicating that the P_death increase does not simply result from the increase of death numbers over time, but it depends also on death positions. C. Simulation of a video with a constant number of death events with a spatially random distribution. Note that the corresponding P_death measurements are constant over time. D. Simulation of a video with a constant number of death events with a clustered distribution. Note that the corresponding P_death measurements are constant over time, but higher than in C.

(PNG)