pone.0295290.s001.tif (1.88 MB)
Sex ratio in mature animals across the selected FF generation.
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posted on 2023-12-21, 18:31 authored by Mathieu Legras, Giulia Ghisleni, Léna Regnard, Manon Dias, Rabouant Soilihi, Enzo Celmar, Guillaume BalavoineFF(2–8)G are the selected generation for which significant numbers of matures have been obtained. “poly” is the control culture with polymorphic worms raised with the same density and food regime as the last three selected FF generations.
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synchronized sexual maturationrequiring air bubblingnervous system organizationlimited water changeindefinite life cycleartificial moonlight regimemedian reproductive agelow worm densityfast cycling culturedevelopmental stages makesadapted culturing methodreverse genetics modelplatynereis dumerilii culture methoddumerilii worm carereproductive biologydevelopmental biologyp model animalannelid modelvast branchvarious fieldstraditional protocolseveral advantagessemelparous reproductionrelatively longrapid growthphylogenetic positionmarine annelidlophotrochozoans addimportant factorsgained popularityfull protocolevolving genomeevolutionary significanceepigenetic regulationcell typescas9 knockoutsbiological rhythms
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