pone.0262616.g001.tif (154.89 kB)
Sequencing outcome for four drug resistance genes between individual and pooled sequenced samples.
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posted on 2022-01-14, 18:26 authored by Swarnali Louha, Camelia Herman, Mansi Gupta, Dhruviben Patel, Julia Kelley, Je-Hoon M. OH, Janani Guru, Jean F. Lemoine, Michelle A. Chang, Udhayakumar Venkatachalam, Eric Rogier, Eldin TalundzicSequencing success (blue dot) or failure (orange dot) is determined by measuring the codon coverage of high-quality reads obtained. Sequencing method (individual vs pooled) is shown on the x axis and parasitemia levels (parasites/μL) are shown on the y axis. Individual sequenced samples = 100 total samples; same samples are pooled at 10:1 ratio = total of 10 sequenced sample pools.
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values (+/- 1time saving approachproviding allele frequencymedian codon coveragedried blood spotsdiv >< pcontinued chloroquine sensitivitycompared codon coverageμl blood showeddrug resistant markersplasmodium falciparum μl blood priorsequencing large numbersdeep sequencing yieldedlower parasite densityaf estimation may0 ct valuepooled sequencing approachdrug resistance genesseveral individual samplesμl priordrug resistancedeep sequencingsequencing successlarge numbergenes (<mdr1 dhps dhfr crt similar realrapid genotypingpooled samplesparasite dnaoften neededmolecular surveillancemixed infectionslow amountsindividual samplesgrouping samplescould leadconferring mutationsbased studiesassociated mutationsalternative costaf calls40 parasites
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