ppat.1009257.s004.tif (252.48 kB)

Sequence alignment of major HIV-1 clades and CRFs proximal to residue 465 in Env.

figure

posted on 2021-02-08, 19:17 authored by Tysheena P. Charles, Samantha L. Burton, Prabhu S. Arunachalam, Christopher A. Cottrell, Leigh M. Sewall, Venkata S. Bollimpelli, Sailaja Gangadhara, Antu K. Dey, Andrew B. Ward, George M. Shaw, Eric Hunter, Rama R. Amara, Bali Pulendran, Marit J. van Gils, Cynthia A. Derdeyn

The amino acid sequence alignment contains BG505.SOSIP.664.gp140 (immunogen), BG505.W6M.Env.C (the parental PV), the BG505.T465N mutant, and a consensus for HIV-1 group M, all major HIV-1 clades and CRFs, with HXB2 as a reference sequence (Genbank K03455). The alignment was generated using https://www.hiv.lanl.gov/content/sequence/NEWALIGN/align.html and https://www.hiv.lanl.gov/content/sequence/SeqPublish/seqpublish.html. N-linked glycan motifs within this region are highlighted in gray and the T465N mutation is indicated in with red text. Dashes indicate conserved residues, while differences are shown, except within the 465 adjacent region, where glycan motifs are indicated by showing the amino acid residues (NXS/T where X is any residue except proline). Dots indicate a gap.

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