pone.0247134.g003.tif (666.13 kB)
Separation of the His-pVP2 particles by sucrose gradient.
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posted on 2021-02-16, 18:38 authored by Carla Marusic, Charifa Drissi Touzani, Alessio Bortolami, Marcello Donini, Claudia Zanardello, Chiara Lico, Emile Rage, Siham Fellahi, Mohammed El Houadfi, Calogero Terregino, Selene Baschieria) ELISA with an anti-VP2 antibody of the sucrose gradient fractions. The fractions were numbered from 1 (F1; top of the tube) to 11 (F11; bottom of the tube). C-: p19 plant extract; C+: inactivated IBDV. b) TEM images of sucrose gradient fractions. In F1 only SVP (T = 1) are present, indicated by the arrows. F2 contains bigger particles with a diameter ranging from 35 to 50 nm (T = 7). In F3 the majority of the particles are VLP with a diameter of ~ 60 nm, (T = 13), but tubular structures are also visible.
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novel subunit vaccinesInfectious Bursal Disease Virusform supramolecular structuresBursal Disease Virusspecific-pathogen-free chicksIBDV strainIBDV neutralizing antibodiesintramuscular immunizationantibodyvirus-like particlesGumboro diseaseVP 2Nicotiana benthamianainsect cellscauses mortalityhistomorphological alterationscapsid protein VP 2transmission electron microscopyVP 2 proteinsucrose density gradient fractionsetiological agentVP 2-based particles400 nmpoultry industry
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