Semi-quantitative scale developed to assess morphological changes following treatment of zebrafish embryos with an amino-terminal fragment of nApoE4_1-151.

Rubric for developmental abnormalities was accomplished in 48 hpf zebrafish following 24-hour treatment with 25 μg/ml of nApoE41-151. This scale was established to quantify the effects of nApoE4_1-151 as compared to untreated, control embryos. Identified hallmark defects that appeared consistently following treatment with nApoE4_1-151 included inflation of pericardial cavity, enlarged hearts, pigmentation alterations, and delays or lack of development in ear and brain structures. Data are representative of 10 embryos treated with 25 μg/ml nApoE4_1-151 per trial for a total of 30 embryos.