Schematics of the reporter constructs generated for MIR172A-E.

The NVG coding sequence is depicted by a short bright red box (NLS), a yellow box (Venus), and a blue box (GUS). This coding sequence was used to replace the hairpin region (dark red and orange lines) in each MIR172 gene. The beige boxes represent exons, gray lines represent introns, and black lines represent intergenic sequences including untranslated regions. Brown boxes indicate adjacent genes. At3g55513 is annotated as encoding a hypothetical protein, whereas At3g08495 and At2g08215 are annotated as expressing long noncoding RNAs, respectively, and were therefore included in the reporter constructs for MIR172A and MIR172D as they may contain regulatory elements relevant to their expression. The complete genomic structure has only been determined for MIR172A and MIR172B. Therefore, the most upstream transcription start site for MIR172C and MIR172E described in [90] were used. RNA-seq data available from Araport [91] were used to estimate which regions surrounding the hairpin coding regions of MIR172A-C are transcribed. Black arrows beneath the schematics indicate ATGs upstream of the NVG reporter, whereas gray arrows indicate ATG that were present before the MIR172 hairpin coding region that were omitted from the reporter constructs. ATG, adenine, thymine, guanine; GUS, β-glucuronidase enzyme; NVG, NLS-Venus-GUS; RNA-seq, RNA-sequencing.

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