Gki156s1.jpg (28.27 kB)
Schematic representations of DNA duplexes formed by oligonucleotides that contain nicks and/or gaps at regular intervals
figure
posted on 2011-12-30, 23:24 authored by Tumpa Sarkar, Christine C. Conwell, Lilia C. Harvey, Catherine T. Santai, Nicholas V. HudCopyright information:
Taken from "Condensation of oligonucleotides assembled into nicked and gapped duplexes: potential structures for oligonucleotide delivery"
Nucleic Acids Research 2005;33(1):143-151.
Published online 07 Jan 2005
PMCID:PMC546144.
© 2005, the authors ©
() The pairing of the 42mer oligonucleotides N1 and N2 to produce a nicked-DNA duplex with nicks in the backbone at every 21 bp. () The formation of gapped-DNA, with 2 nt gaps at every 21 bp, by the pairing of the 44mer oligonucleotides G1 and G2. () The formation of nicked-gapped-DNA, with alternating nicks and 2 nt gaps at every 21 bp, by the pairing of the 42mer oligonucleotide N1 and the 44mer oligonucleotide G2. Oligonucleotide sequences are given in Materials and Methods.