Schematic representation of the Spike glycoprotein and the rare mutations highlighted by NGS.

Cartoon describing the SARS-CoV-2 Spike glycoprotein 3D structure and its interaction with the cellular plasma membrane together with a graphical representation of Spike-related sequencing data. RBD: RNA binding domain; NTD: N-terminal domain; FP: fusion protein; TA: transmembrane anchor; IT: intracellular tail; S1: subunit 1; S2: subunit 2; HR1: heptad repeat region 1; HR2: heptad repeat region 2; the inverted black triangle represents the amino acid bridge. Created with Biorender.com.