pone.0293838.g001.tif (117.79 kB)
Schematic representation of α0-thal--SEA,--THAI and--CR type deletions on α-globin gene cluster and designated primers.
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posted on 2023-11-06, 18:25 authored by Chedtapak Ruengdit, Manoo Punyamung, Nutjeera Intasai, Sakorn PornprasertAbbreviations: SEA, Southeast Asian; CR, Chiang Rai; F, Forward primer; R, Reverse primer; WT, Wild-type; kb, kilobases.
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specific peak heightssea ), thaidiv >< pclearly identify individuals65 17650 17640 17604 176mean melting temperaturetube multiplex real89 %, 0resolution melting analysishigh prevalence areashrm analysis indicatedcr sup19 %, respectivelyresolution meltinghigh prevalencesingle tubexlink ">type deletionstime pcrroutine testingroutine settingrisk couplesrequire simplemultiple deletionsmethod wouldhydrops fetalishighly accurateglobin genechiang raicarrier screeningamplified fragmentsaccurate tests135 samples--< sup
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