pone.0297884.g001.tif (152.62 kB)
Schematic of tissue processing.
figure
posted on 2024-03-01, 18:26 authored by Omar Almomani, James Nnamutete, Zhongtian Shao, Victoria Menya Biribawa, HenryRoger Ssemunywa, Annemarie Namuniina, Brenda Okech, Sofya Ulanova, David Zuanazzi, Cindy M. Liu, Aaron A. R. Tobian, Ronald M. Galiwango, Rupert Kaul, Jessica L. ProdgerSamples were divided equally, with one portion processed for flow cytometry immediately and one cryopreserved for 2–5 days before processing. Both samples were digested to a single cell suspension by mechanical and enzymatic digestion (collagenase) in the presence of DNAse. Cell suspensions were filtered to remove debris, stained with fluorescently labeled antibodies against markers of interest, and analyzed using an LSRII flow cytometer (HI-FBS: heat-inactivated FBS). Created with BioRender.com.
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restricts immune analysesnegative males agedmucosal tissue likeleast 60 %,flow cytometry phenotypingflow cytometry analysiscompared fresh analysisalthough expression levelscell populations relevanteight foreskin sampleswhole tissue cryopreservationforeskin flow cytometryreduces hiv acquisitionflow cytometrycell expressionsurgical acquisitionanalyzed freshcell subsetsinner foreskinforeskin tissuestimepoints immediatelysignificant impactremaining tissuesrelative rankingpowerful toollater thawingincompletely understoodimmediately cryopreservedhiv susceptibilityhinders research18 years
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