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Schematic of the reporter construct generated for APETALA2 and RNA-seq data for AP2-LIKE gene transcript levels in Col and mir172abd mutants.

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posted on 2021-02-02, 18:25 authored by Diarmuid S. Ó’Maoiléidigh, Annabel D. van Driel, Anamika Singh, Qing Sang, Nolwenn Le Bec, Coral Vincent, Enric Bertran Garcia de Olalla, Alice Vayssières, Maida Romera Branchat, Edouard Severing, Rafael Martinez Gallegos, George Coupland

(A) The Venus coding sequence is depicted by a yellow box. This coding sequence was fused in frame to the final exon of AP2 by removing the stop codon of AP2. The beige boxes represent exons, gray lines represent introns, and black lines represent intergenic sequences including untranslated regions. Brown boxes indicate adjacent genes. At4g09445/At4g09415 are annotated as long noncoding RNAs, and At4g09415 is annotated as “other RNA” and were therefore included in the reporter construct as they may contain regulatory elements important for AP2 expression. (B, C) Graphs depicting RNA-seq-derived data of the mRNA levels in apices of SMZ, SNZ, TOE1, TOE3, and AP1 in Col-0 and miR172abd at the indicated time points after germination in (B) LDs and (C) SDs. Error bars represent the standard deviation of 3 biological replicates. Data underlying panels (B) and (C) are provided in S1 Data. AP2, APETALA2; AP2-LIKE, APETALA2-LIKE; LD, long-day; RNA-seq, RNA-sequencing; SD, short-day.

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