pone.0256846.g006.tif (86.73 kB)
Schematic drawing of the reporter plasmid construct.
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posted on 2021-09-10, 17:37 authored by Lara M. Hochfeld, Marta Bertolini, David Broadley, Natalia V. Botchkareva, Regina C. Betz, Susanne Schoch, Markus M. Nöthen, Stefanie Heilmann-HeimbachThe WNT10A promoter (1,641 bp) was cloned into pGL-3-basic using XhoI and HindIII. Using In-Fusion® HD-cloning the EBF1 binding site (EBF1-BS) (93 bp) containing the rs3856551 C- or T-allele was inserted between the BamHI and SalI restriction sites of pGL-3-basic.
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luciferase reporter assayshaploreg database researchhair shaft formationexact molecular mechanismsdiv >< passociated variants exertunderlying pathophysiological mechanismregulatory annotations revealedmpb risk locusmpb risk allelempb lead varianttranscription factor ebf12q35 risk locusproves functional interaction>/ ebf1 interactionmale pattern baldnesspattern baldnessfunctional mechanismfunctional interactionregulatory effectregulatory architecturempb pathophysiologympb developmentpotential interactionebf1 activatesebf1 /<whose genevitro sheds lightpreviously reportedpredominant relevancefrequently observedfirst followexperiments confirmeventually resultsbinding siteassociation signal2q35 maps
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