Fig 6.tif (1.18 MB)

Scaff10-8 is not cytotoxic.

figure

posted on 2018-01-26, 18:29 authored by Katharina Schrade, Jessica Tröger, Adeeb Eldahshan, Kerstin Zühlke, Kamal R. Abdul Azeez, Jonathan M. Elkins, Martin Neuenschwander, Andreas Oder, Mohamed Elkewedi, Sarah Jaksch, Karsten Andrae, Jinliang Li, Joao Fernandes, Paul Markus Müller, Stephan Grunwald, Stephen F. Marino, Tanja Vukićević, Jenny Eichhorst, Burkhard Wiesner, Marcus Weber, Michael Kapiloff, Oliver Rocks, Oliver Daumke, Thomas Wieland, Stefan Knapp, Jens Peter von Kries, Enno Klussmann

(A) Western blot showing protein expression of AKAP-Lbc and RhoA in five different cell lines and in primary rat inner medullary collecting duct (IMCD) cells: All cells express full-length AKAP-Lbc (308 kDa) and shorter splice variants. RhoA (22 kDa) is also expressed in all cells. MW = molecular weight. (B) Cytotoxicity was assessed after 24–72 h of incubation with Scaff10-8 (3, 30, 100 μM). Staurosporine as inducer of apoptosis was used as a positive control. Absorption was determined at 450 nm and was related to medium absorption (blank). DMSO was used as a control. Normalised to untreated cells after 24 h, three independent experiments with four data points each are shown. n = 3. Statistically significant differences were determined applying one-way ANOVA with posthoc Bonferroni. Mean ± SEM is plotted.