SMO and GPR161 localization in response to Hh pathway stimulation.

Normalized fluorescence intensity (au) of A) SMO and B) GPR161 with and without pathway stimulation. Large symbol = median normalized fluorescence intensity for individual coverslip data. Small symbol = normalized fluorescence intensity of one cilium. Measurement from three separate batches are indicated by a unique symbol shape and color. Total number of cilia measured listed in the second row below the graph. Representative images are shown below graphs for unstimulated (-SAG) and stimulated (+SAG) cells. Two-tailed Student’s t-test was performed. P-values represented significant differences if they ranged from 0.01 to 0.05 (*), 0.001 to 0.01 (**), and <0.001 (***).