SEC for the isolation of full-length S1P₁ and GPR3 monomers analyzed by SDS-PAGE and Coomassie-staining.

S1P₁ and GPR3 monomers after SEC with and without addition of lipids into the SEC running buffer (25 mM HEPES pH 7.5; 500 mM NaCl; 10% (v/v) Glycerol; 0.5 mg/ml DDM; 0.01 mg/ml CHS)) are shown. The samples were loaded after concentration with less than 2% of the total bed volume and run with 1 mL/min. F represents the first SEC fraction with monomer as main compound within the sample.