pntd.0009696.g006.tif (364.25 kB)
SEA stimulate ROS production of macrophages by NADPH oxidase and mitochondria.
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posted on 2021-08-16, 17:32 authored by Yanxiong Yu, Junling Wang, Xiaohong Wang, Pan Gu, Zhigang Lei, Rui Tang, Chuan Wei, Lei Xu, Chun Wang, Ying Chen, Yanan Pu, Xin Qi, Beibei Yu, Xiaojun Chen, Jifeng Zhu, Yalin Li, Zhijie Zhang, Sha Zhou, Chuan Su(A) J774A.1 cells were pretreated with Syk inhibitor R406 and then stimulated with PBS or 40 μg/m SEA for 24 hours. Cells were collected and whole cell lysates were extracted for detecting Syk, p-Syk, and Nox2 by immunoblotting. GAPDH was used as an internal control. (B) J774A.1 cells were stimulated with PBS or 40 μg/m SEA for 24 hours. Mitochondrial membrane potentials and ROS in cells were measured by FCM analysis using TMRM and MitoSOX, respectively. Data are representative of two independent experiments, each with triplicates.
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nicotinamide adenine dinucleotide p...ROS productionegg-induced granulomatous responseReactive oxygen speciesM 2 macrophage differentiationM 2 macrophages-rich granulomasSchistosome eggsNOXschistosomiasis japonica-induced he...reactive oxygen species productionhepatic granulomatous responsesM 1 macrophage differentiationNADPHM 2 macrophagesfibrosisjaponicum egg antigens
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