SEA induce high production of ROS in macrophages to facilitate M2 macrophage differentiation.

J774A.1 cells were pretreated with Apo and then stimulated with 40 μg/m SEA for 24 hours. (A) ROS production in J774A.1 cells from each group was detected by FCM using the ROS-sensitive probe DCFH-DA. (B and C) Expression of CD16/32 (M1) and CD206 (M2) on J774A.1 cells were detected by FCM analysis. Representative histograms were shown (B). The bar graphs show the average percentages of CD16/32⁺ M1 or CD206⁺ M2 macrophages (C). Data are means ± SD of triplicate cultures and representative of three independent experiments. ***P<0.001. NS indicating not significant.