pone.0248007.g005.tif (374.01 kB)
SARS-CoV-2 spike-specific T cells responses analyzed by ELISpot.
figure
posted on 2021-03-22, 17:23 authored by Eakachai Prompetchara, Chutitorn Ketloy, Kittipan Tharakhet, Papatsara Kaewpang, Supranee Buranapraditkun, Teerasit Techawiwattanaboon, Suwitra Sathean-anan-kun, Patrawadee Pitakpolrat, Supaporn Watcharaplueksadee, Supaporn Phumiamorn, Wassana Wijagkanalan, Kanitha Patarakul, Tanapat Palaga, Kiat RuxrungthamMice splenocytes were simulated with pooled peptides from S1 (A) or S2 (B) regions. Each bar represents mean of IFN- γ spot forming unit (SPF) per million splenocytes responses to each peptide pool. (C) Sum of IFN- γ responses in mice (N = 5) immunized with different DNA vaccine constructs. Mann-Whitney test was used to compare the different in the total IFN- γ responses from each group. (D) Ex vivo cytokines expression collected from the culture supernatant after splenocytes from S-immunized group were stimulated with spike pooled peptides for 24 h.
