ppat.1009878.s004.tiff (7.3 MB)

SARS-CoV-2-mediated cytokine and chemokine production is not induced upon PBMC stimulation with UV-inactivated virus.

figure

posted on 2021-09-02, 17:25 authored by Martina Severa, Roberta A. Diotti, Marilena P. Etna, Fabiana Rizzo, Stefano Fiore, Daniela Ricci, Marco Iannetta, Alessandro Sinigaglia, Alessandra Lodi, Nicasio Mancini, Elena Criscuolo, Massimo Clementi, Massimo Andreoni, Stefano Balducci, Luisa Barzon, Paola Stefanelli, Nicola Clementi, Eliana M. Coccia

Peripheral blood mononuclear cells (PBMC) were left untreated (not stimulated, ns) or stimulated for 24 hours with infectious or UV-inactivated (UV-I) SARS-CoV-2 (CoV2, MOI = 0.04). Production of cytokines (IL-6 and TNF-α) (A) and chemokines (CXCL-10, CXCL-9, CCL-2, CXCL-8 and CCL-5) (B) was tested by multiparametric cytokine bead arrays in collected cell culture supernatants. Shown results were mean relative values ± SEM of 3 independent experiments. P-values were depicted as follows: *p≤0.05; ** p≤0.001.

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