ppat.1009395.s006.tif (401.65 kB)

S6 Fig -

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posted on 2021-03-08, 18:40 authored by Michael G. Dorrington, Clinton J. Bradfield, Justin B. Lack, Bin Lin, Jonathan J. Liang, Tregei Starr, Orna Ernst, Julia L. Gross, Jing Sun, Alexandra H. Miller, Olivia Steele-Mortimer, Iain D. C. Fraser

A) WT, Rnf31+/-, Rbck1-/-, and Sharpin-/- RAW264.7 cells were infected with wt J2315, as in Fig 5. After 20h, cells were lysed and lysates were cultured for 24 h before enumerating colonies; n = 5 per condition, representative of 3 independent experiments. B) The same cell lines were pre-stimulated with rIFNβ or PBS for 24 h before infecting with dsRed+ J2315 (MOI = 1). Bacterial growth was measured using live-cell high-content imaging based on dsRed fluorescence. Data presented as fold-change in dsRed fluorescence intensity compared to t = 3 h p.i. C) RT-PCR showing effective knockdown of LGALS3BP or p62 in Fig 5F. mRNA is shown relative to cells transfected with a non-target control (NTC) siRNA. * = p≤0.05, ** = p≤0.01, *** = p≤0.001, and **** = p≤0.0001 by Mann-Whitney U test.

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