S5 Fig -

A. Hierarchical clustering analysis of effects of itaconate and DI on IAV-infected dTHP-1 cells. dTHP1 cells were infected with IAV (PR8M, MOI = 1) and gene expression assessed by microarray analysis 12 h p.i. Analysis based on the same microarray data as used for Figs 3 and S4, using the top 100 DEGs (FDR F-test <1.89E-09). Uninfected cells and untreated IAV-infected cells form one clade and itaconate and DI treated IAV-infected cells the other clade. From top to bottom the brackets identify the following clades: (i) a large clade of DEGs that are upregulated upon itaconate treatment, (ii) DEGs that are upregulated by both itaconate and DI, (iii) a predominantly pro-inflammatory clade that is induced in IAV infection and globally downregulated by both treatments, (iv) DEGs downregulated by both itaconate and DI, (v) DEGs downregulated only by itaconate, (vi) DEGs upregulated only by DI. B. Enrichment plots based on the 99 genes contained in GO term Response to type I interferon, based on the microarray data used for Figs 3, S4, and S5A. Enrichment scores are plotted on the y-axis, the mRNAs (identified by vertical lines) along the x-axis, ranked by fold change. The plots illustrate pronounced enrichment in a large number of genes due to infection, which is nearly quantitatively depleted by treatment with both itaconate and DI.

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