ppat.1009395.s005.tif (528.71 kB)
S5 Fig -
figure
posted on 2021-03-08, 18:40 authored by Michael G. Dorrington, Clinton J. Bradfield, Justin B. Lack, Bin Lin, Jonathan J. Liang, Tregei Starr, Orna Ernst, Julia L. Gross, Jing Sun, Alexandra H. Miller, Olivia Steele-Mortimer, Iain D. C. FraserA) WT and Ifnar1-/- iBMDMs were infected with live or heat-killed Bc at an MOI of 10 while oxygen consumption and the pH of the culture media was measured on a Seahorse XF analyzer. Proton efflux rate was enumerated over the course of 7.5 h. B, C) WT and Ifnar1-/- iBMDMs were infected with live or heat-killed Bc at given MOIs or else stimulated with 1000 U/mL of rIFNβ for 24 h. H2O2 was measured using a luminescence-based assay. B) shows luminescence levels while C) shows luminescence relative to unstimulated cells. All experiments were performed with n = 5, and are shown as representative of 2 independent experiments *** = p≤0.001 by Mann-Whitney U test.
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