ppat.1009395.s003.tif (619.09 kB)

S3 Fig -

figure

posted on 2021-03-08, 18:39 authored by Michael G. Dorrington, Clinton J. Bradfield, Justin B. Lack, Bin Lin, Jonathan J. Liang, Tregei Starr, Orna Ernst, Julia L. Gross, Jing Sun, Alexandra H. Miller, Olivia Steele-Mortimer, Iain D. C. Fraser

A, B) WT and Ifnar1^-/- iBMDMs were infected with A) dsRed+ Bc (MOI = 1) or B) GFP+ STm (MOI = 10) for 22 h. Supernatants were removed, and levels of glucose 6-phosphate dehydrogenase were measured. Percent cell lysis was enumerated as compared to uninfected cells lysed with lysis buffer. C, D) WT, Casp1/11-/-, and Ifnar1-/- BMDMs were infected with wt J2315 for the indicated times before supernatants were removed and cells were lysed. C) Lysates were analyzed by Western blot for Caspase-1, Gasdermin-D, GAPDH, and RhoGDI (loading control). D) IL-1β was measured in supernatants by ELISA. 1 mM Leu-Leu-O-methyl (LLoM) and 10 μM nigericin (Nig) were used as positive controls of inflammasome activation. E) WT, Casp1/11-/-, and Ifnar1-/- BMDMs were infected with wt J2315 and then live-imaged in the presence of Draq7 after 3 h of infection. Draq7 uptake was measured for 19 h. *** = p≤0.001, **** = p≤0.0001 by Mann-Whitney U test.

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