S2 Fig -

(A) Immunohistochemistry for PDF and GFP reveals the expression of GFP in the l-LNvs of c929-GAL4/UAS-gfp flies but not in the brains of c929-GAL4/UAS-gfp; Cry-Gal80 flies. (B) Pdfr⁵⁵⁰⁴; PDF>/UAS-Pdfr^WT flies exhibit more waking during the WMZ than Pdfr⁵³⁰⁴; Pdf-GAL4/+ and Pdfr⁵³⁰⁴;UAS-Pdfr^WT/+ parental controls (ANOVA F_[2,91] = 4.63, p = 0.01 n = 41–64 flies/genotype) flies. Data underlying this figure can be found in S8 Data. GFP, green fluorescent protein; l-LNv, large ventral lateral neuron; PDF, pigment dispersing factor; WMZ, wake maintenance zone.

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