ppat.1009425.s006.tif (545.53 kB)
Rhamnolipid biosynthesis gene rhlA shows altered expression profiles in P. aeruginosa PA14 ΔtatABC and ΔpetA mutants compared with wild type and fail to respond to exogenous PQS.
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posted on 2021-08-30, 17:38 authored by Eliza Ye-Chen Soh, Frances Smith, Maxime Rémi Gimenez, Liang Yang, Rebecca Munk Vejborg, Matthew Fletcher, Nigel Halliday, Sophie Bleves, Stephan Heeb, Miguel Cámara, Michael Givskov, Kim R. Hardie, Tim Tolker-Nielsen, Bérengère Ize, Paul WilliamsBioluminescence from a chromosomal rhlA’-lux fusion as a function of growth (RLU/OD) over time when introduced into (A) the PA14 wild type, (B) ΔtatABC and (C) ΔpetA mutants in the absence or presence of exogenous PQS (20 μM).
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wild type levelsreduced bioluminescence revealedreduced antibiotic tolerancedependent quorum sensingdependent autoinduction resultingdiv >< pdefective biofilm architecture>< sub ><primary signalling defectidentify factors requiredpseudomonas aeruginosa tat prevented pqs>< sub xmlnsedna deficient biofilmsaeruginosa tat aeruginosa dependent qsp biofilm maturationtat tat substratesub xmlnsrieske sub>rhla pqse pqsa pqs phza1 lux cytc cytb 1 transposon librarysulfur subunitrieske ironqs ).parent strainmembrane vesiclemajor constituentgenetic complementationgenes resultsextracellular matrixextracellular dnaexogenous pqsenhanced susceptibilitydriven autoarginine translocationalso phenocopied34 validated
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