pone.0293838.s001.tif (103.48 kB)
Result of conventional Gap-PCR method for α0-thal--SEA,--THAI and--CR heterozygote DNA samples.
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posted on 2023-11-06, 18:25 authored by Chedtapak Ruengdit, Manoo Punyamung, Nutjeera Intasai, Sakorn PornprasertM: 100 bp DNA ladder, Lane 1: Normal DNA sample, Lane 2: α0-thal--SEA heterozygote, Lane 3: α0-thal--THAI heterozygote, Lane 4: α0-thal--CR heterozygote.
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specific peak heightssea ), thaidiv >< pclearly identify individuals65 17650 17640 17604 176mean melting temperaturetube multiplex real89 %, 0resolution melting analysishigh prevalence areashrm analysis indicatedcr sup19 %, respectivelyresolution meltinghigh prevalencesingle tubexlink ">type deletionstime pcrroutine testingroutine settingrisk couplesrequire simplemultiple deletionsmethod wouldhydrops fetalishighly accurateglobin genechiang raicarrier screeningamplified fragmentsaccurate tests135 samples--< sup
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