Residues critical for B. subtilis RodA function and their location in a model of the B. subtilis RodA structure predicted by AlphaFold2.

Mutability of critical residues of B. subtilis RodA were categorized based on previous Mutseq analysis [5]. Dominant-negative E. coli FtsW mutations isolated in this study that alter identical residues in B. subtilis RodA are indicated in parentheses. Residues critical for B. subtilis function are mapped to a model of RodA and the residues of FtsW whose substitutions displayed a dominant-negative effect are mapped to a model of FtsW. Red: immutable residues; magenta: residues that tolerate only conservative changes (dominant-negative mutations in FtsW); pink: residues with limited mutability.

(TIF)