1471-2172-8-7-4.jpg (38.14 kB)

Representative flow cytometry scatter plots demonstrated the analytical integrity of the field-processed mid-mission samples

figure

posted on 2011-12-31, 07:26 authored by Brian Crucian, Pascal Lee, Raymond Stowe, Jeff Jones, Rainer Effenhauser, Raymond Widen, Clarence Sams

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Taken from "Immune system changes during simulated planetary exploration on Devon Island, high arctic"

http://www.biomedcentral.com/1471-2172/8/7

BMC Immunology 2007;8():7-7.

Published online 23 May 2007

PMCID:PMC1890299.

For flow cytometry analysis, whole blood was preserved with a non-cross linking preservative. This reagent was found to inhibit cellular auto fluorescence, as well as preserve antigenic structure of both extracellular as well as intracellular (cytokine) molecules. CD14 vs. side scatter plots allowing enumeration of granulocyte, monocyte and lymphocyte subsets are presented derived from a CD45+ gate to eliminate artifactual debris.